Asepsis is currently not working under Mavericks. It's, in my knowledge, the only single-purpose app existing for this job. Therefore, what is the less intrusive solution to prevent .DS_Store creation after the update to Mavericks?
Asepsis updates have been officially released for Mavericks compatibility - http://asepsis.binaryage.com/
I've personally tested it, working like a charm!
The free app TinkerTool 5.0, which has many functions, can be used on Mavericks to disable creating .DS_Store files on network volumes, but I do not believe this has any effect on .DS_Store files on your local Mac.
BlueHarvest does not prevent the creation of .DS_Store files, but it can delete them automatically.
You can also delete .DS_Store files by using find:
find / -name .DS_Store -delete